The ministry said Russian air power had supported Syrian troops in freeing the town of Abu Kamal from Isis, and that "facts of direct cooperation and support provided by the US-led coalition to the ISIS terrorists" came to light during the operation.
The ministry said US-led coalition planes instead tried to impede Russian Aerospace Forces operations in the area. Russia said the Americans actively interfered with Russian airstrikes, to provide cover for the Isis fighters.
"The US are actually covering the [fleeing] ISIS combat units to recover their combat capabilities, redeploy, and use them to promote the American interests in the Middle East", the Ministry added.
The allegations are extremely grave, but may be harder to take seriously given the "irrefutable proof" offered in the form of photographic accompaniment. The image went viral nearly immediately, but it was quickly debunked once someone recognized that the image was actually from a 2015 trailer for AC-130 Gunship Simulator: Special Ops Squadron.
The Russian Defense Ministry made a fool of itself today when it posted on Twitter and Facebook "irrefutable evidence" that the U.S. aided ISIS, which turned out to be screengrabs from a well-known video game and a video published online by the Iraqi military in 2016.
AFP was able to compare the images in the emailed defence ministry statement with the images of the videogame on YouTube and confirm the resemblance.
A cached version of the original statement can still be viewed online.
The military stood by its allegations saying "the refusal of United States command to inflict strikes on ISIL convoys on November 9 is an established fact recorded in the transcript of conversations".
Eliot Higgins, a researcher with Kings College London and Atlantic Council's Digital Forensic Research Lab, pointed out this was not the first time the Russian Defense Ministry's social media accounts were caught lying.
The Kremlin denied that Putin had shown the wrong footage to the American director Oliver Stone during one of a series of interviews. The footage appeared to be an exact match for old footage of USA forces in Afghanistan.
A US Department of Defense spokesman said this incident was just the latest "episode" by the Russians.
The Russian military is investigating claims that a civilian employee attached the bogus images, state-run media reported later Tuesday.